![]() ![]() Great trackers make it easy to keep tabs on your cat. The Best Trackers Have Functional, Intuitive Apps or Receivers. The best cat trackers have long battery lives, reliable connections, and ranges large enough to help you find your cat, whether he’s lounging on the porch or exploring blocks away. With a reliable, accurate tracker, you’ll know when your cat strays from home, where he’s gone, and which direction you need to go to find him. ![]() Though they’re not a substitute for microchipping and close supervision, cat trackers can help you keep tabs on your cat when he’s out of sight. Reliable, Accurate Tracking Is Essential. We tried the trackers with different types of collars to gauge their comfort and security. To evaluate the cat trackers on this list, we performed in-depth testing to determine how easy they were to set up and how well they performed in various real-world applications. The cat trackers we tested were purchased at full retail price and the entire testing process was funded by without direct input or influence from the companies involved. Most of the products on this list have been tested in-depth by our Head of Content, Mallory, with her two cats, Wessie and Forest. We spent hours researching the different types of cat trackers, exploring the options on the market, and reading hundreds of customer reviews. In addition to our own product research and testing, we’ve read hundreds of customer reviews and consulted veterinary experts and cat behaviorists for insight into what to look for when evaluating these products.īased on extensive research, hands-on testing, and expert insights, we selected the following nine cat trackers as the best you can buy. From automatic litter boxes to pet fountains and feeders, our experts have spent hundreds of hours testing these products’ performance, user experience, and cat-friendliness. Over the last several years, we’ve written in-depth reviews on dozens of pet tech solutions. Before we review the Tractive GPS Cat LTE + Tracker and the rest of our top recommendations, let’s go over a few of the things you need to look for in a tracker. ![]()
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![]() Joseph on the Brandywine Catholic Church in Wilmington, Del. (AP Photo/Manuel Balce Ceneta) President Joe Biden and first lady Jill Biden walk between tombstones to attend Mass at St. ![]() "I know this is a very tragic day for you, but I wanted you to know that all of us here at the White House were thinking about you and praying for you and also for your two children," Nixon said. President Joe Biden on Saturday commemorated the 49th anniversary of the car crash that killed his first wife and infant daughter, visiting their graves at. Sunday marks the 50th anniversary of the car crash that killed Biden’s first wife Neilia Hunter Biden and 13-month-old daughter Naomi. Shortly after the crash, then-President Richard Nixon called Joe Biden, according to a recording at Nixon's presidential library. Their dad took his oath as a senator by their hospital bedsides. "And my two boys, but for the jaws of life and a rescue crew saving their life, would not have been around either."īeau and Hunter Biden were seriously injured after the wreck. "My wife was killed and my daughter was killed," he continued then. Joe Biden and first wife, Neilia, with sons Beau and Hunter, cut his 30th birthday cake at a party in Wilmington, Delaware on NovemThe Bidens car was hit by a truck the week. Over the years, the former vice president has spoken openly about the terrible car crash that killed his wife Neilia and their 1-year-old daughter Naomi and injured their two sons, Hunter and. ![]() A tractor trailer hit your wife and your three children while they were shopping,'" Biden recalled at an event in Newton, Iowa, during his 2020 campaign. She was so nervous, she said, 'You gotta come home. They put a pretty young woman on the phone. Joe Biden’s first wife, Neilia Hunter, whom he married in 1966, was killed in a car crash in 1972, along with their 13-month-old daughter Naomi, leaving Joe to care for his two young sons, Beau. "I was down in Washington hiring staff and I got a phone call from a first responder. (AP) President Joe Biden and his family held a private memorial service Sunday to mark the 50th anniversary of the car crash that killed his first wife and their baby daughter. The 1972 crash, weeks after he won his first Senate race, also partially defined his career in Congress. ![]() The death of Joe Biden's wife, daughter and older son have long shaped his approach to grief and empathy, he has said. ![]() ![]() Below is how to update HP Bluetooth driver for Windows 10 through Device Manager: Apart from updating, the tool can also be used to reinstall Bluetooth drivers. What’s more, you can use this in-built program to update outdated device drivers. Way 2: Update HP Laptop Bluetooth Driver in Device Managerīy default, Windows comes with a utility tool called Device Manager that shows graphical representations of hardware and related drivers. ![]() ![]() Once everything is done, restart your HP laptop.Īlso Read: Toshiba Bluetooth Driver Download and Update for Windows PC Thereafter, open the downloaded file and comply with the on-screen instructions to complete the HP Bluetooth driver installation.
![]() Once you complete the steps, the system will download images from the cloud and set them in rotation on the Lock screen of Windows 11. In the “Personalize your lock screen” setting, select the Windows Spotlight option. ![]() To enable the Lock screen Spotlight on Windows 11, use these steps: Change Lock screen with Spotlight on Windows 11 Use the “Turn off the screen after the slideshow has played for” option and select the time before turning off the display.Īfter you complete the steps, the Windows 11 Lock screen will show and rotate the images from the folder locations you chose. (Optional) Under the “Advanced slideshow settings” section, check the “Include camera roll folders from this PC and OneDrive” option. Select the folder with the images to rotate in the Lock screen. (Optional) Click the Remove button for the default slideshow locations. In the “Personalize your lock screen” setting, select the Slideshow option. To enable the Lock screen slideshow on Windows 11, use these steps: Change Lock screen with slideshow on Windows 11 Once you complete the steps, the Lock screen will apply the settings and show the new background image. (Optional) Clear the “Get fun facts, tips, tricks, and more on the lock screen” option. Select the image to use as a background in the Windows 11 Lock screen. Step 1: Long-tap on the home screen and select Wallpaper and style. In the “Personalize your lock screen” setting, select the Picture option. Here’s how you can enable the same on your Galaxy S24 device. To change the Lock screen wallpaper on Windows 11, use these steps:Ĭlick the Lock screen page on the right side.
![]() ![]() That was far from the end of the saga, though. More Questions Emerge Credit: ElenaR/ Shutterstock Summy Company with the now-famous birthday lyrics. Then, in 1935, the Hills registered their work through the Clayton F. The lawsuit was settled and the Hills were eligible for payment whenever the song was used. When it appeared in Irving Berlin’s 1933 Broadway musical As Thousands Cheer, however, Mildred and Patty’s youngest sister, Jessica, stepped in and filed a court case saying her family was owed royalties. Patty later said, “I was never a money-grubber.” But the Hills never copyrighted the “Happy birthday” version of the lyrics. The tune soon grew in popularity and started to appear more in print. How exactly that happened is unknown, but by 1924, it appeared in another songbook edited by Robert Coleman with the Hill sisters’ original lyrics as the first verse and “Happy birthday to you” as the second. Over time, the lyrics changed and the tune began to be used as a celebratory birthday song - the version that we know today. The Song Began to Morph Credit: JFunk/ Shutterstock They included it in a songbook, Song Stories for Kindergarten, which they published in 1893. Together, the Hill sisters wrote the song “Good Morning to All.” Three of the four lines were just that, while the third line was “Good morning, dear children.” “She was the musician and I was, if it is not using too pretentious a word, the poetess," Patty said of their process in 1934, adding that Mildred, who also taught, would perfect the melody by trying it out on her young students. While she was also a composer and performer, the elder Hill focused her musical studies on Negro spirituals, often writing about the subject using the pen name “Johnan Tonsor.” Hill, born in 1859, was just as forward-thinking in the world of musicology. ![]() Instead of structured learning, she championed a more natural method of kindergarten focused on children’s instincts and creativity. A Pair of Innovative Sisters Wrote the Song Credit: Africa Studio/ Shutterstockīorn in Kentucky in 1868, Patty Smith Hill was known for breaking the mold with early childhood education. But the way that it spreads so organically is what made the tune’s copyright a subject of debate that was only legally settled in 2015. The song has been passed down for generations, with many people learning the tune just by listening to family and friends sing it at parties. Yet for something so straightforward, the celebratory tune has a surprisingly complicated history. With only six notes, six words, and four lines - three of them the same - “Happy Birthday” is one of the most universal songs on the planet. ![]() ![]() ![]() Numbers - Square, Cube, Square Root and Cubic Root CalculatorĬalculate square, cube, square root and cubic root. Water - Density, Specific Weight and Thermal Expansion Coefficientsĭefinitions, online calculator and figures and tables with water properties like density, specific weight and thermal expansion coefficient of liquid water at temperatures ranging 0 to 360☌ (32 to 680☏). Thermal conductivity coefficients for insulation materials, aluminum, asphalt, brass, copper, steel, gases and more. Solids, Liquids and Gases - Thermal Conductivities Modulus of Elasticity) and Ultimate Tensile Strength and Yield Strength for materials like steel, glass, wood and many more. Young's Modulus, Tensile Strength and Yield Strength Values for some Materials Calculate angular velocity.Ĭalculate miter saw protractor angles for skirting and decorative mouldings work.įriction - Friction Coefficients and Calculatorįriction theory with calculator and friction coefficients for combinations of materials like ice, aluminum, steel, graphite and many more. Thermal properties of water at different temperatures like density, freezing temperature, boiling temperature, latent heat of melting, latent heat of evaporation, critical temperature and more. Psychrometric chart for air at barometric pressure 29.921 inches of Mercury and temperature ranging 20 oF to 120 oF.įree online UTM to Latitude and Longitude coordinates converter. When a material is stretched in one direction it tends to get thinner in the other two directions.Īir - Psychrometric Chart for Standard Atmospheric Conditions - Imperial Units Hot Air Balloons - Calculate Lifting WeightsĬalculate hot air ballons lifting forces. ![]() Linear temperature expansion coefficients for common materials like aluminum, copper, glass, iron and many more. Thermal Expansion - Linear Expansion Coefficients Thermal properties of air at different temperatures - density, viscosity, critical temperature and pressure, triple point, enthalpi and entropi, thermal conductivity and diffusivity and more. Calculating and sizing steel pipe thermal expansion loops. ![]() ![]() ![]()
![]() cerevisiae strain containing spytag at the C terminal of the targeted protein is constructed and the fixed cells are reacted with spycatcher-oligo to covalently attach DNA oligo to targeted proteins in situ. The single-cell protein abundance can be quantified from the sequencing results. After protein identification, the protein-oligo conjugates are recovered and the oligo part is amplified by PCR to generate next-generation sequencing libraries. Then the cells are pooled together, analyzed by gel electrophoresis to identify protein and post-translational modifications. As a proof-of-concept experiment, we used this strategy to measure histone protein (H2B) and its monoubiquitination isoform (H2BK123ub) in single yeast cells.Ī The cells are uniquely barcoded by oligo tagging. Therefore, iDentification/qUantification SeparaTion (DUET) could combine the powers of conventional biochemical analysis methods for protein identification and next-generation sequencing for protein quantification. The barcodes from each protein species can be quantified by next-generation sequencing (NGS) to determine the abundance of those proteins in single cells (Fig. Despite pooling, the single-cell identities of each protein molecule are preserved in the barcodes. Then different protein species, such as target protein and its PTM could be identified by conventional biochemical analysis (e.g. Specifically, after barcoding proteins from single cells with oligonucleotides, we pool a large number of cells together to yield enough material. We reasoned that if the identification and quantification of single-cell protein analysis could be separated into two distinct steps, we could overcome the limitations of conventional biochemical techniques. Here, we reported an alternative approach to accurately detect protein and its PTM isoforms in single cells. Recently, great advances have been made in single-cell mass spectrometry analysis using reporter ions and cell pooling 7 with potential for further increases in sensitivity, throughput, and coverage. Antibody-based methods rely on the availability of good affinity reagents which are often the limiting factor in experiments 6. Conventional protein-detection methods, such as western blots, enzyme-linked immunosorbent assay (ELISA) are generally difficult to downscale to the single-cell level to detect such heterogeneities 5. Protein and its post-translational modification (PTM) states are often involved in dynamic and oscillatory processes that are not synchronized in a population and show substantial single-cell heterogeneities 1, 2, 3, 4. Our results revealed the heterogeneities of H2B ubiquitination levels in single cells from different cell-cycle stages, which is obscured in ensemble measurements. We used this strategy, iDentification and qUantification sEparaTion (DUET), to measure histone protein H2B and its monoubiquitination isoform, H2Bub, in single yeast cells. We barcode the proteins from single cells by tagging them with oligonucleotides, pool barcoded cells together, run bulk gel electrophoresis to separate protein and its PTM isoform and quantify their abundances by sequencing the oligonucleotides associated with each protein species. Here, we develop a strategy to detect targeted protein and its PTM isoforms in single cells. Unlike nucleic acids, proteins cannot be amplified, making detection of minute quantities from single cells difficult. All rights reserved.While technologies for measuring transcriptomes in single cells have matured, methods for measuring proteins and their post-translational modification (PTM) states in single cells are still being actively developed. The proposed method represents an affordable, accurate and reproducible approximation that could be used in the presence of limited resources availability.īackground subtraction Densitometry ImageJ Quantification Western blot.Ĭopyright © 2018 Elsevier B.V. In this work, we describe the use of office scanner coupled with the ImageJ software together with a new image background subtraction method for accurate Western blot quantification. Furthermore, the use of semi-quantitative software as ImageJ (Java-based image-processing and analysis software) is clearly increasing in different scientific fields. Due to the technical knowledge required for densitometry analysis together with the resources availability, standard office scanners are often used for the imaging acquisition of developed Western blot films. Western blot quantification method constitutes a critical step in order to obtain accurate and reproducible results. It constitutes a multistep method that allows the detection and/or quantification of proteins from simple to complex protein mixtures. Since its first description, Western blot has been widely used in molecular labs. ![]() ![]() In such event, you must destroy all copies of Save Wizard For PS4 License Key in your possession and remove any installation thereof.ĭata Power Development reserves the right to terminate a users license including but not limited to the following reasons: Without prejudice to any other rights, Data Power Development Limited may terminate this EULA if you fail to comply with the terms and conditions of this EULA. This includes but is not limited to games, cheats, Advanced Mode or re-signing. (h) Changes to the product and/or contents.ĭata Power Development Limited reserves the right to add or change or remove or limit features and or content without notice. ![]() You may not use Save Wizard for the purposes of monetary gain, in particular but not limited to creating modified saves for sale. You must comply with all applicable laws regarding use of Save Wizard For PS4. Any supplemental software code provided to you as part of the Support Services shall be considered part of Save Wizard For PS4 and subject to the terms and conditions of this EULA. You may not rent, lease, or lend the Save Wizard For PS4 in part or whole.ĭata Power Development Limited may provide you with support services related to Save Wizard For PS4 ("Support Services"). You may not reverse engineer, decompile, or disassemble Save Wizard For PS4, except and only to the extent that such activity is expressly permitted by applicable law, notwithstanding this limitation. (c) Prohibition on Reverse Engineering, Decompilation, and Disassembly. ![]() You may not distribute registered copies of Save Wizard For PS4 to third parties. You must not remove or alter copyright notices on any and all copies of Save Wizard For PS4. DESCRIPTION OF OTHER RIGHTS AND LIMITATIONS. You may also make copies of Save Wizard For PS4 as may be necessary for backup and archival purposes.Ģ. Save Wizard For PS4 is licensed as follows:ĭata Power Development Limited grants you the right to a limited number of installs and the use of authorized copies of Save Wizard For PS4 on your computer running a validly licensed copy of the operating system for which Save Wizard For PS4 was designed i.e. Save Wizard For PS4 is licensed, not sold. Save Wizard For PS4 is protected by copyright laws, international copyright treaties, and other intellectual property laws and treaties. ![]() If you do not agree to the terms of this EULA, do not install or use Save Wizard For PS4. This license agreement represents the entire agreement concerning Save Wizard For PS4 between you and Data Power Development Limited, (referred to as "licenser"), and it supersedes any prior proposal, representation, or understanding between the parties. By installing or otherwise using Save Wizard For PS4, you agree to be bound by the terms of this EULA. IMPORTANT PLEASE READ THE TERMS AND CONDITIONS OF THIS LICENSE AGREEMENT CAREFULLY BEFORE CONTINUING WITH THIS PROGRAM INSTALL: Data Power Development Limited End-User License Agreement ("EULA") is a legal agreement between you (either an individual or a single entity) and Data Power Development Limited for the Save Wizard For PS4 which may include associated software components, media, printed materials, and "online" or electronic documentation. Once a purchase is made, A refund can not be issued for any reason.īy purchasing, You also agree to the END-USER LICENSE AGREEMENT FOR SAVE WIZARD FOR PS4. By purchasing, you agree to all of the following īe sure this is what you want before purchasing. ![]() ![]() No, each redeem code can typically be used only once per Fortnite account. Q1:Can I use the redeem codes multiple times? Please note that while some codes may have expiration dates, resulting in the loss of the opportunity to obtain certain exclusive or limited-time cosmetics for free, these items might still be available for purchase in the Fortnite item shop at a later date. Players can enter the code to acquire the Croft Manor spray, showcasing their connection to the iconic Tomb Raider character. Initially available for a limited time in late March, the code appears to be working again as of July 2021. However, codes shared by Epic Games or content creators can generally be used by more players.Description: To celebrate the crossover event featuring Lara Croft, Epic Games released a code that unlocked her special spray. If you purchase a code for a cosmetic item or V-Bucks, you will only be able to redeem it once. These codes are mostly limited and last for a few hours. Additionally, Epic Games and content creators sometimes release codes for cosmetic items. Just like V-Bucks, you may obtain codes online or in stores. How can I get a Fortnite code for a skin? You can buy physical V-Bucks codes at retailers, but you may also purchase codes on Amazon, G2A, and other reputable websites. Due to this, make sure to redeem it as quickly as possible! Fortnite codes FAQ Where can I purchase Fortnite V-Bucks codes? The emote will expire in March and is limited to 25 million uses. At the moment, there is one code you can redeem following the steps we listed above: If you’re looking for active Fortnite codes for free rewards, you’ve come to the right place. Additionally, some codes may be available for redemption in PlayStation and Xbox Store from Sony and Microsoft respectively. If you are on a PC, you may also redeem Fortnite codes through Epic Games Launcher. Choose the platform you play Fortnite on.Here is how to redeem a Fortnite V-Bucks card: In case you have a V-Bucks code and want to redeem it, the process is slightly different, but you still need to do it on the Epic Games website. ![]() How to redeem a V-Bucks gift card in Fortnite You can get XP, free cosmetics, and free rewards for a limited time in modes like LEGO Fortnite with these access codes. Keep an eye on new codes that are mostly released by content creators on X (formerly Twitter). If you’d like to try it out, check out how to get the Nanner Ringer emote, which is obtained through Fortnite’s redeem codes. Using this method, you can redeem various cosmetics, from outfits to emotes. To see it and use it in your Epic account, launch Fortnite and check your locker. If the code is valid, you will get a prompt to accept the item. Once you confirm the alpha-numeric code redemption, the cosmetic item will be added to your Fortnite account. Enter the cosmetic item code (case-sensitive).Open /redeem and click your name in the upper right corner.This process is the same for all systems, whether you have a gaming console like Nintendo Switch or PlayStation or a PC. If you have a code for an in-game reward in Fortnite, you need to redeem it on /redeem. Image via Epic Games How to redeem codes in Fortnite In this case, your friend will need to log in to their account through internet browser. You may also send Fortnite codes as gifts. ![]() If you have multiple accounts, this can be a problem, so log in with the right account before you begin the process. This step is crucial, so make sure you double-check it. Check the link above for all active Fortnite codes.īefore you begin, make sure you are logged into the correct Epic Games account. Additionally, you may perform it on any device, from a PC to your mobile phone. The process is very simple and takes less than a minute. You may use the code redemption for various goodies, from cosmetic items to V-Bucks. To redeem Fortnite codes, you will have to open the video game’s website. If you want to obtain items directly from the shop, however, check out the latest Fortnite Item shop updates. ![]() Besides cosmetic items, we’ll also show you how to redeem V-Bucks codes. ![]() Our comprehensive guide will lead you through the entire redemption process. If you’d like to know how to redeem codes in Fortnite, you’ve come to the right place. However, Fortnite codes cannot be redeemed in the game, as this process has to be done on Epic’s website. Epic Games made the code redemption straightforward, and by using it, you can add cosmetic items and V-Bucks to your account. Redeeming Fortnite codes is a relatively simple process. ![]() |
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